FGF9 anticorps (Biotin)
Aperçu rapide pour FGF9 anticorps (Biotin) (ABIN115847)
Antigène
Voir toutes FGF9 (FGF-9) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
-
-
Specificité
- Recognises Fibroblast Growth Factor 9 (FGF-9).
-
Purification
- Affinity chromatography.
-
Immunogène
- Highly pure (>98%) recombinant mFGF-9.
-
-
-
-
Indications d'application
-
ELISA: (Direct): To detect mFGF-9 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2- 0.4 ng/well of recombinant mFGF-9. (Sandwich): To detect mFGF-9 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine FGF-9 (PP002P) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mFGF-9. Western Blot: To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mFGF-9 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
-
-
-
Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
-
Buffer
- PBS, pH 7.2 without preservatives.
-
Agent conservateur
- Without preservative
-
Conseil sur la manipulation
- Avoid repeated freezing and thawing.
-
Stock
- 4 °C/-20 °C
-
Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
-
-
- FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))
-
Autre désignation
- FGF9
-
Sujet
- FGF9 is a heparin binding growth factor, which is a member of the FGF family of proteins. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is produced mainly by neurons and may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.Synonyms: Fibroblast growth factor 9, GAF, Glia-activating factor, HBGF9, Heparin-binding growth factor 9
-
ID gène
- 14180
-
NCBI Accession
- NP_038546
-
UniProt
- P54130
Antigène
-